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De novo assembly of bacterial transcriptomes from RNA-seq data

Author:
Tjaden, Brian
Source:
GenomeBiology.com 2015 v.16 no.1 pp. 1
ISSN:
1465-6906
Subject:
algorithms, bacteria, computer software, genes, high-throughput nucleotide sequencing, transcriptome
Abstract:
Transcriptome assays are increasingly being performed by high-throughput RNA sequencing (RNA-seq). For organisms whose genomes have not been sequenced and annotated, transcriptomes must be assembled de novo from the RNA-seq data. Here, we present novel algorithms, specific to bacterial gene structures and transcriptomes, for analysis of bacterial RNA-seq data and de novo transcriptome assembly. The algorithms are implemented in an open source software system called Rockhopper 2. We find that Rockhopper 2 outperforms other de novo transcriptome assemblers and offers accurate and efficient analysis of bacterial RNA-seq data. Rockhopper 2 is available at http://cs.wellesley.edu/~btjaden/Rockhopper .
Agid:
1201357