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Sphingobacterium gobiense sp. nov., isolated from soil of the Gobi Desert

Zhao, Peng, Zhou, Zhengfu, Chen, Ming, Lin, Wenna, Zhang, Wei, Wei, Gehong
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 12 pp. 3931-3935
DNA, Sphingobacterium, fatty acids, microbiology, nucleotide sequences, pH, phylogeny, ribosomal RNA, sequence homology, sodium chloride, soil, temperature, China, Gobi Desert
A Gram-stain-negative, short rod-shaped, non-motile, non-spore-forming bacterial strain, designated H7 ᵀ, was isolated from the Gobi desert, Xinjiang Province of China. The temperature, NaCl and pH ranges for growth were 8–40 °C (optimum 30 °C), 0–5 % (w/v) NaCl and pH 6–10 (optimum pH 7), respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H7 ᵀ belonged to the genus Sphingobacterium and showed highest sequence similarity (91 %) to Sphingobacterium composti DSM 18850. The genomic DNA G+C content was 44.3 mol%. MK-7 was identified as the predominant respiratory quinone. Strain H7 ᵀ contained C ₁₆ : ₁ω7 c and/or C ₁₆ : ₁ω6 c , iso-C ₁₅ : ₀ and iso-C ₁₇ : ₀ 3-OH as major fatty acids. These data support the affiliation of strain H7 ᵀ to the genus Sphingobacterium while phenotypic data indicated it is a representative strain of a novel species for which the name Sphingobacterium gobiense sp. nov. is proposed. The type strain is H7 ᵀ (= ACCC 05757 ᵀ = KCTC 32293 ᵀ).