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Sphingobacterium gobiense sp. nov., isolated from soil of the Gobi Desert

Author:
Zhao, Peng, Zhou, Zhengfu, Chen, Ming, Lin, Wenna, Zhang, Wei, Wei, Gehong
Source:
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 12 pp. 3931-3935
ISSN:
1466-5034
Subject:
DNA, Sphingobacterium, fatty acids, microbiology, nucleotide sequences, pH, phylogeny, ribosomal RNA, sequence homology, sodium chloride, soil, temperature, China, Gobi Desert
Abstract:
A Gram-stain-negative, short rod-shaped, non-motile, non-spore-forming bacterial strain, designated H7 ᵀ, was isolated from the Gobi desert, Xinjiang Province of China. The temperature, NaCl and pH ranges for growth were 8–40 °C (optimum 30 °C), 0–5 % (w/v) NaCl and pH 6–10 (optimum pH 7), respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H7 ᵀ belonged to the genus Sphingobacterium and showed highest sequence similarity (91 %) to Sphingobacterium composti DSM 18850. The genomic DNA G+C content was 44.3 mol%. MK-7 was identified as the predominant respiratory quinone. Strain H7 ᵀ contained C ₁₆ : ₁ω7 c and/or C ₁₆ : ₁ω6 c , iso-C ₁₅ : ₀ and iso-C ₁₇ : ₀ 3-OH as major fatty acids. These data support the affiliation of strain H7 ᵀ to the genus Sphingobacterium while phenotypic data indicated it is a representative strain of a novel species for which the name Sphingobacterium gobiense sp. nov. is proposed. The type strain is H7 ᵀ (= ACCC 05757 ᵀ = KCTC 32293 ᵀ).
Agid:
1203404