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Marinobacterium mangrovicola sp. nov., a marine nitrogen-fixing bacterium isolated from mangrove roots of Rhizophora mangle

Alfaro-Espinoza, Gabriela, Ullrich, Matthias S.
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 12 pp. 3988-3993
DNA, Marinobacterium, Rhizophora mangle, catalase, flagellum, genes, nitrogen-fixing bacteria, nucleotide sequences, pH, phylogeny, ribosomal RNA, roots, sequence homology, sodium chloride
A nitrogen-fixing marine bacterium, designated strain Gal22 ᵀ, was isolated from mangrove roots of Rhizophora mangle . Cells were Gram-stain-negative rods, motile with a single polar flagellum. Growth was observed at 4–42 °C, pH 5.5 to 10 and with 0–18 % (w/v) NaCl. Strain Gal22 ᵀ was positive for catalase and oxidase. Q-8 was the predominant lipoquinone. The DNA G+C content was 57.0 mol%. Based on phylogenetic analysis of 16S rRNA gene, strain Gal22 ᵀ belongs to the genus Marinobacterium . The closely related strains were shown to be Marinobacterium lutimaris DSM 22012 ᵀ and Marinobacterium litorale IMCC1877 ᵀ with 99 % and 96 % 16S rRNA gene sequence similarity, respectively. DNA–DNA relatedness analysis indicated that strain Gal22 ᵀ was different from M. lutimaris DSM 22012 ᵀ. On the basis of genotypic, morphological and biochemical characteristics, a novel species, Marinobacterium mangrovicola sp. nov. (type strain, Gal22 ᵀ = DSM 27697 ᵀ = CIP 110653 ᵀ), is proposed.