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Microbacterium petrolearium sp. nov., isolated from an oil-contaminated water sample

Wang, Huimin, Xiang, Tingsheng, Wang, Yanwei, Song, Jinlong, Zhai, Yi, Chen, Xiaorong, Li, Yanting, Zhao, Bingqiang, Zhao, Bin, Ruan, Zhiyong
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 12 pp. 4168-4172
DNA, Microbacterium, bacteria, cell walls, fatty acids, galactose, glycolipids, hydrolysates, nucleic acid hybridization, nucleotide sequences, oil fields, ornithine, pH, quinones, ribose, ribosomal RNA, sequence analysis, sodium chloride, temperature, China
A Gram-staining-positive, rod-shaped, aerobic bacterium, designated strain LAM0410 ᵀ, was isolated from an oil-contaminated water sample from the Dagang Oilfield, PR China. The temperature and pH ranges for growth were 4 °C to 50 °C and pH 4.4 to pH 12.0, respectively. The strain did not need NaCl for growth, but could tolerate a concentration of up to 12 % (w/v). Cell wall hydrolysates from the isolate showed that the diamino acid was ornithine. The cell wall sugars contained ribose and galactose. The glycan moiety of the cell wall contained N -glycolyl residues. The major respiratory quinones were MK-10, MK-11 and MK-12. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and nine unknown glycolipids. The major fatty acids were anteiso-C ₁₅ : ₀, anteiso-C ₁₇ : ₀ and iso-C ₁₆ : ₀. 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Microbacterium and was closely related to Microbacterium sediminis MCCC 1A06153 ᵀ and Microbacterium murale DSM 22178 ᵀ with 97.5 % and 97.4 % sequence similarities, respectively; while the DNA–DNA hybridization values were 33.1±3.4 % and 21.8±1.6 %, respectively. The DNA G+C content was 67.4 mol%, as determined by the T ₘ method. Based on its phenotypic and genotypic properties, strain LAM0410 ᵀ represents a novel species of the genus Microbacterium , for which the name Microbacterium petrolearium sp. nov. is proposed; the type strain is LAM0410 ᵀ (= ACCC 00719 ᵀ = JCM 19612 ᵀ).