Jump to Main Content
Microbacterium petrolearium sp. nov., isolated from an oil-contaminated water sample
- Wang, Huimin, Xiang, Tingsheng, Wang, Yanwei, Song, Jinlong, Zhai, Yi, Chen, Xiaorong, Li, Yanting, Zhao, Bingqiang, Zhao, Bin, Ruan, Zhiyong
- International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 12 pp. 4168-4172
- DNA, Microbacterium, bacteria, cell walls, fatty acids, galactose, glycolipids, hydrolysates, nucleic acid hybridization, nucleotide sequences, oil fields, ornithine, pH, quinones, ribose, ribosomal RNA, sequence analysis, sodium chloride, temperature, China
- A Gram-staining-positive, rod-shaped, aerobic bacterium, designated strain LAM0410 ᵀ, was isolated from an oil-contaminated water sample from the Dagang Oilfield, PR China. The temperature and pH ranges for growth were 4 °C to 50 °C and pH 4.4 to pH 12.0, respectively. The strain did not need NaCl for growth, but could tolerate a concentration of up to 12 % (w/v). Cell wall hydrolysates from the isolate showed that the diamino acid was ornithine. The cell wall sugars contained ribose and galactose. The glycan moiety of the cell wall contained N -glycolyl residues. The major respiratory quinones were MK-10, MK-11 and MK-12. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and nine unknown glycolipids. The major fatty acids were anteiso-C ₁₅ : ₀, anteiso-C ₁₇ : ₀ and iso-C ₁₆ : ₀. 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Microbacterium and was closely related to Microbacterium sediminis MCCC 1A06153 ᵀ and Microbacterium murale DSM 22178 ᵀ with 97.5 % and 97.4 % sequence similarities, respectively; while the DNA–DNA hybridization values were 33.1±3.4 % and 21.8±1.6 %, respectively. The DNA G+C content was 67.4 mol%, as determined by the T ₘ method. Based on its phenotypic and genotypic properties, strain LAM0410 ᵀ represents a novel species of the genus Microbacterium , for which the name Microbacterium petrolearium sp. nov. is proposed; the type strain is LAM0410 ᵀ (= ACCC 00719 ᵀ = JCM 19612 ᵀ).