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Genomic organization and promoter analysis of a transcriptional repressor gene from Fenneropenaeus chinensis
- Lai, Xiaofang, Shen, Shanrui, Gao, Huan, Yan, Binlun
- Molecular biology reports 2015 v.42 no.2 pp. 393-398
- Fenneropenaeus chinensis, TATA box, White spot syndrome virus, binding sites, exons, immune response, introns, luciferase, molecular cloning, reporter genes, shrimp, transcription (genetics), transcription factors
- In this study, we cloned and sequenced genomic sequences from a Fenneropenaeus chinensis transcriptional repressor gene, FcTR. The FcTR gene is 2,671 bp in length and has four exons and three introns. The 873 bp promoter contains several transcription factor binding sites, including a TATA box and a cyclic AMP-responsive element. Promoter deletion analysis using a luciferase reporter gene identified regulatory elements. Challenge with white spot syndrome virus increased expression from the promoter-deletion constructs. These results suggest that FcTR might play an important role in the shrimp immune response.