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Complete genomic sequence of a Pineapple mealybug wilt-associated virus-1 from Hainan Island, China

Yu, Naitong, Luo, Zhiwen, Fan, Hongyan, Zhang, Zhili, Li, Xianghong, Wang, Jianhua, Liu, Zhixin, He, Fan
European journal of plant pathology 2015 v.141 no.3 pp. 611-615
Ananas comosus, Pineapple mealybug wilt-associated virus 1, RNA-directed RNA polymerase, coat proteins, genome, heat shock response, nucleotides, open reading frames, pineapples, proteinases, sequence analysis, trees, China, Hawaii
This is the first report of the complete genome sequence of Pineapple mealybug wilt-associated virus-1 (PMWaV-1 HN, KJ872494) identified on pineapple [Ananas comosus (L.) Merr.] in Hainan island, China. The genome is 13,069 nucleotides long, possessing seven open reading frames (ORFs). The ORF1 encodes a 233 kDa polyprotein which has PRO protease domain, MTR methyltransferase domain and HEL helicase domain; The ORF2 ~ ORF7 encode RNA-dependent RNA polymerase protein (RdRp), a 6 kDa protein (p6), heat shock 70 homolog protein (HSP70h), a 46 kDa protein (p46), coat protein (CP) and a 24 kDa protein (p24), respectively. Full-length nucleotide sequence analysis of PMWaV-1 HN genome confirmed it 97.99 % homology with PMWaV-1 (AF414119) which was isolated from Hawaii, USA. Furthermore, based on Maximum Likelihood method, all conserved trees of RdRp, HSP70h and CP proteins sequences revealed that PMWaV-1 HN together with other PMWaV-1 isolates clearly separated into PMWaV-1 group.