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A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue

Author:
Xia, Xiaohu, Xu, Ye, Ke, Rongqin, Zhang, Heng, Zou, Mingqiang, Yang, Wei, Li, Qingge
Source:
Analytical and bioanalytical chemistry 2013 v.405 no.23 pp. 7541-7544
ISSN:
1618-2642
Subject:
chloramphenicol, detection limit, europium, immunoassays, inhibitory concentration 50, milk, quantitative analysis
Abstract:
A europium nanoparticle-based lateral flow immunoassay for highly sensitive detection of chloramphenicol residue was developed. The detection result could be either qualitatively resolved with naked eye or quantitatively analyzed with the assistance of a digital camera. In the qualitative mode, the limit of detection (LOD) was found to be 0.25 ng/mL. In the quantitative mode, the half-maximal inhibition concentration (IC50) was determined to be 0.45 ng/mL and the LOD can reach an ultralow level of 0.03 ng/mL, which is ~100 times lower than that of the conventional colloidal gold-based lateral flow immunoassay. Potential application of the established method was demonstrated by analyzing representative cow milk samples.
Agid:
1206204