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Multiclass method for antimicrobial analysis in animal feeds by liquid chromatography–tandem mass spectrometry

Author:
Borràs, S., Companyó, R., Guiteras, J., Bosch, J., Medina, M., Termes, S.
Source:
Analytical and bioanalytical chemistry 2013 v.405 no.26 pp. 8475-8486
ISSN:
1618-2642
Subject:
acetonitrile, anti-infective agents, antibiotic residues, cattle feeds, feed contamination, ionization, liquid chromatography, methanol, poultry, solid phase extraction, swine, tandem mass spectrometry
Abstract:
A rapid multiclass method that covers 50 antimicrobials from 13 different families in animal feeds was developed. Samples were extracted using a mixture of methanol, acetonitrile and a McIlvaine buffer combined with sonication. Feed extracts were simply diluted prior to injection, since the clean-up strategies that were tested, based on either solid-phase extraction or dispersive solid-phase extraction, were ineffective at minimizing matrix-related signal suppression/enhancement. Analysis was carried out by liquid chromatography coupled to tandem mass spectrometry using an electrospray ionization source operating in positive and negative modes. For the quantification, matrix-fortified standard calibration curves were used to compensate for matrix effects and losses in sample preparation. The method was validated in-house in pig, poultry and cattle feed matrices and showed satisfactory performance characteristics. Thus, the proposed approach was suitable for application in a routine high-throughput laboratory for the official control of feeds.
Agid:
1206584