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Improving Enzymatic Hydrolysis of Corn Stover Pretreated by Ethylene Glycol-Perchloric Acid-Water Mixture

He, Yu-Cai, Liu, Feng, Gong, Lei, Lu, Ting, Ding, Yun, Zhang, Dan-Ping, Qing, Qing, Zhang, Yue
Applied biochemistry and biotechnology 2015 v.175 no.3 pp. 1306-1317
biomass, biotechnology, cellulosic materials, corn stover, cost effectiveness, enzymatic hydrolysis, ethanol, ethylene, glucose, hydrolysates, lignocellulose, microwave radiation, saccharification, temperature
To improve the enzymatic saccharification of lignocellulosic biomass, a mixture of ethylene glycol-HClO₄-water (88.8:1.2:10, w/w/w) was used for pretreating corn stover in this study. After the optimization in oil-bath system, the optimum pretreatment temperature and time were 130 °C and 30 min, respectively. After the saccharification of 10 g/L pretreated corn stover for 48 h, the saccharification rate was obtained in the yield of 77.4 %. To decrease pretreatment temperature and shorten pretreatment time, ethylene glycol-HClO₄-water (88.8:1.2:10, w/w/w) media under microwave irradiation was employed to pretreat corn stover effectively at 100 °C and 200 W for 5 min. Finally, the recovered hydrolyzates containing glucose obtained from the enzymatic hydrolysis of pretreated corn stovers could be fermented into ethanol efficiently. These results would be helpful for developing a cost-effective pretreatment combined with enzymatic saccharification of cellulosic materials for the production of lignocellulosic ethanol.