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Gene expression profiling of hybridoma cells after bursal-derived bioactive factor BP5 treatment

Feng, Xiu L., Liu, Qing T., Cao, Rui B., Zhou, Bin, Li, De Y., Zhang, Yuan P., Liu, Ke, Liu, Xiao D., Wei, Jian C., Qiu, Ya F., Li, Xin F., Ma, Zhi Y., Chen, Pu Y.
Amino acids 2012 v.43 no.6 pp. 2443-2456
T-lymphocytes, antibody formation, bioactive properties, bursa of Fabricius, cell differentiation, cell-mediated immunity, cytokines, gene expression, gene expression regulation, genes, hybridomas, immunomodulation, luciferase, microarray technology, neoplasm cells, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, signal transduction
Bursa of Fabricius is the acknowledged vital humoral immune system for B cell differentiation and antibody production. To study the molecular mechanism underlying the effect of bursal-derived BP5, we used gene microarray to analyze the genomic expression profiling of BP5-treated hybridoma cells. BP5 exhibited an immunomodulatory effect on antibody production in hybridoma cells and induced alterations in the gene expression profiles related to the immune-related biological processes, such as T cell activation and proliferation, B cell activation, B cell-mediated immunity, and cytokines cytokine production involved in immune response. In addition, 26 biological pathways associated with immunomodulatory functions were regulated in BP5-treated hybridoma cells, in which p53 signal pathway played an important role in antitumor. Among these regulated genes, 12 differentially expressed genes were verified by qRT-PCR. The activation of p53 activity by BP5 was further confirmed by p53 luciferase reporter assay and p53 expression. Our data revealed that bursal-derived BP5 could regulate various immune-related cellular processes, including antitumor factor p53 signal pathway, perhaps partially accounting for the reported immunomodulatory roles and novel antiproliferation on tumor cells functions of bursal-derived bioactive factor BP5.