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Paludibacter jiangxiensis sp. nov., a strictly anaerobic, propionate-producing bacterium isolated from rice paddy field

Author:
Qiu, Yan-Ling, Kuang, Xiao-Zhu, Shi, Xiao-Shuang, Yuan, Xian-Zheng, Guo, Rong-Bo
Source:
Archives of microbiology 2014 v.196 no.3 pp. 149-155
ISSN:
0302-8933
Subject:
DNA, acetates, arabinose, bacteria, catalase, cellobiose, fatty acids, fermentation, fructose, galactose, glucose, iron, lactose, maltose, mannose, new species, nitrates, nucleotide sequences, pH, pectins, phosphatidylethanolamines, propionic acid, ribosomal RNA, rice, sequence analysis, starch, sucrose, sulfur, thiosulfates, xylose, yeast extract
Abstract:
A mesophilic, obligately anaerobic, propionate-producing fermentative bacterium, designated strain NM7ᵀ, was isolated from rural rice paddy field. Cells of strain NM7ᵀare Gram-negative, non-motile, non-spore-forming, short rods, and negative for catalase. The strain grew optimally at 37 °C (the range for growth 15–40 °C) and pH 7.0 (pH 5.0–7.5). The strain could grow fermentatively on various sugars, including arabinose, xylose, fructose, galactose, glucose, mannose, cellobiose, lactose, maltose, sucrose, pectin and starch. The main end products of glucose fermentation were acetate and propionate. Yeast extract was not required but stimulated the growth. Nitrate, sulfate, thiosulfate, elemental sulfur, sulfite, and Fe(III) nitrilotriacetate were not used as terminal electron acceptors. The G+C content of genomic DNA was 42.8 mol%. The major cellular fatty acids were C₁₅:₀, anteiso-C₁₅:₀, C₁₆:₀, and C₁₇:₀. The most abundant polar lipid of strain NM7ᵀwas phosphatidylethanolamine. 16S rRNA gene sequence analysis revealed that it belongs to the family Porphyromonadaceae of the phylum Bacteroidetes. The closest recognized species was Paludibacter propionicigenes (91.4 % similarity in 16S rRNA gene sequence). A novel species, Paludibacter jiangxiensis sp. nov., is proposed to accommodate strain NM7ᵀ(=JCM 17480ᵀ = CGMCC 1.5150ᵀ = KCTC 5844ᵀ).
Agid:
1209573