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Isolation and Identification of a 10-Deacetyl Baccatin-III-Producing Endophyte from Taxus wallichiana

Li, Yongchao, Yang, Jing, Zhou, Xiuren, Zhao, Wenen, Jian, Zaiyou
Applied biochemistry and biotechnology 2015 v.175 no.4 pp. 2224-2231
Taxus wallichiana var. mairei, Trichoderma, bark, biochemistry, biotechnology, culture media, endophytes, evolution, fungi, high performance liquid chromatography, manufacturing, nuclear magnetic resonance spectroscopy, paclitaxel, taxanes
Endophytic fungi of inner root bark of Taxus wallichiana var. mairei were investigated in order to find endophytes producing 10-DABIII (10-deacetyl baccatin III). Purified colonies were cultured in potato dextrose broth (PDB), and then the organic extracts from fungi were analyzed with HPLC, LC-MS, and¹H NMR. Of 102 fungal endophytes isolated from the inner root bark, only one strain named IRB54 can yield 10-DABIII but no taxol and baccatin III. In PDB culture medium, its productivity was 187.564 ug/l. Based on its morphological characteristics and molecular data, the IRB54 strain was identified as Trichoderma sp. The isolation of the fungus IRB54 yielding 10-DABIII will provide an alternative resource to manufacture taxol/taxotere via semi-synthesis and some useful clues for improving the understanding of taxane synthesis evolution.