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An essential role of a ferritin-like protein in acid stress tolerance of Listeria monocytogenes

Author:
Milecka, Dorota, Samluk, Anna, Wasiak, Katarzyna, Krawczyk-Balska, Agata
Source:
Archives of microbiology 2015 v.197 no.2 pp. 347-351
ISSN:
0302-8933
Subject:
Listeria monocytogenes, gene expression regulation, genes, mutants, osmotic stress, pH, potassium chloride, stress tolerance, temperature, transcription (genetics), transcription factors
Abstract:
The expression of ten genes of Listeria monocytogenes previously identified as penicillin G-inducible was transcriptionally analyzed in the presence of 0.5 M KCl, pH 5.0 and 42 °C. This study revealed that all the genes are upregulated by osmotic stress, seven by acid stress and four by temperature stress conditions. The contribution of a gene encoding a ferritin-like protein (fri), a two-component phosphate-response regulator (phoP) and an AraC/XylS family transcription regulator (axyR) to temperature, acid and osmotic stress tolerance was further examined by analysis of nonpolar deletion mutants. This revealed that a lack of PhoP or AxyR does not affect the ability to grow under the tested stress conditions. However, the Δ fri strain showed slightly delayed growth under osmotic and clearly impaired growth under acid stress conditions, indicating an important role of the ferritin-like protein in acid stress tolerance.
Agid:
1211807