Main content area

Novel polymorphic EST-derived microsatellite markers for the red-listed five needle pine, Pinus dabeshanensis

Zhang, Zhi-Yong, Cui, Bin-Bin, Mao, Jian-Feng, Pang, Xiao-Ming, Liu, Hui, Li, Ying-Yue
Conservation genetics resources 2015 v.7 no.1 pp. 191-192
Pinus, altitude, genetic markers, loci, microsatellite repeats, mountains, transcriptome, China
Pinus dabeshanensis is an endangered five needle pine endemic to mid-South of China. This pine finds its very limited natural occurrence in the Dabie mountains area with altitudes ranging 900–1400 m. We identified 3,516 SSR loci from the transcriptome assembly of the congeneric species P. lambertiana, and evaluated 671 loci we found. Finally, 28 novel polymorphic microsatellite markers were developed for P. dabeshanensis. Markers were characterized using 36 individuals, representing all existing populations from across the species geographic range. Species level analysis of these 28 polymorphic markers revealed the mean number per locus was 2.786 (range 2–6) and mean observed and expected heterozygosities were 0.332 (range 0.000–1.000) and 0.316 (range 0.033–0.691), respectively. This study provides the first set of microsatellite markers for the conservation genetics which in turn will facilitate conservation management for this pine.