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Pseudacidovorax austerolens sp. nov., a nifH bacterium isolated from Himalayan valley soil, India

Tyagi, Shivani, Singh, Dileep Kumar
Annals of microbiology 2015 v.65 no.1 pp. 217-223
Acidovorax, Xylophilus, bacteria, chemotaxonomy, fatty acid composition, fatty acids, new species, nucleic acid hybridization, nucleotide sequences, phylogeny, ribosomal RNA, sequence analysis, soil, India
Gram-negative, rod-shaped bacterial strain ptl-2ᵀ, was isolated from soil of the Himalayan valley, India. The bacterial strain ptl-2ᵀhas been characterized using a polyphasic taxonomic approach including morphological characterization, fatty acid analysis, biochemical tests, 16S rRNA and nifH gene sequence analysis. 16S rRNA gene sequence analysis showed that the strain ptl-2ᵀbelonged to the genus Pseudacidovorax and is closely related to Pseudacidovorax intermedius (99.3 % similarity). It showed <97 % similarity to species of the genera Acidovorax, Alicycliphilus, Xylophilus, Giesbergeria and Simplicispira. The generic assignment has been confirmed on the basis of chemotaxonomic data, which revealed the fatty acid profile, characteristic of the genus Pseudacidovorax, consisting of C₁₆:₀(21.78) and C₁₈:₁w₇c(19.78) as major fatty acids. Phylogenetic, chemotaxonomic and phenotypic analysis based on signature sequences, DNA-DNA hybridization and physiological characterizations, confirms that strain ptl-2ᵀrepresents a different species of the genus Pseudacidovorax for which the name Pseudacidovorax austerolens sp. nov. is proposed. The type strain ptl-2ᵀ(= CCUG 58759ᵀ, = DSM 24877ᵀ) has been submitted to two culture collection centres. GenBank accession numbers for the 16S rRNA and nifH sequence of strain Pseudacidovorax austerolens ptl-2ᵀare FJ581042 and GQ249664, respectively.