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Assessment of morphological changes of Clostridium acetobutylicum by flow cytometry during acetone/butanol/ethanol extractive fermentation

Author:
González-Peñas, Helena, Lu-Chau, Thelmo Alejandro, Moreira, Maria Teresa, Lema, Juan Manuel
Source:
Biotechnology letters 2015 v.37 no.3 pp. 577-584
ISSN:
0141-5492
Subject:
Clostridium acetobutylicum, acetone, butanol, ethanol, fermentation, flow cytometry, olive oil, solvents, vegetative cells
Abstract:
Acetone/butanol/ethanol (ABE) fermentation by Clostridium acetobutylicum was investigated in extractive fed-batch experiments. In conventional fermentations, metabolic activity ceases when a critical threshold products concentration is reached (~21.6 g solvents l⁻¹). Solvents production was increased up to 36.6 and 37.2 g l⁻¹, respectively, using 2-butyl-1-octanol (aqueous to organic ratio: 1:0.25 v/v) and pomace olive oil (1:1 v/v) as extraction solvents. The morphological changes of different cell types were monitored and quantified using flow cytometry. Butanol production in extractive fermentations with pomace olive oil was achieved mainly by vegetative cells, whereas the percentage of sporulating cells was lower than 10 %.
Agid:
1214316