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Isolation of a novel beta-cypermethrin degrading strain Bacillus subtilis BSF01 and its biodegradation pathway

Author:
Xiao, Ying, Chen, Shaohua, Gao, Yuanqi, Hu, Wei, Hu, Meiying, Zhong, Guohua
Source:
Applied microbiology and biotechnology 2015 v.99 no.6 pp. 2849-2859
ISSN:
0175-7598
Subject:
Bacillus subtilis, activated sludge, biodegradation, bioremediation, biotechnology, carbon, cyhalothrin, cypermethrin, deltamethrin, gas-chromatography-mass spectrometry, metabolites, microbiology, pH, pollution, response surface methodology
Abstract:
Continuous use of the pyrethroid insecticide beta-cypermethrin (beta-cp) has resulted in serious environmental contamination problems. We report here that a novel bacterial strain BSF01, which was isolated from activated sludge and identified as Bacillus subtilis (collection number: CCTCC AB 2014103), showed high efficiency in degrading beta-cp. Strain BSF01 was able to utilize beta-cp as the sole carbon source for growth and degraded 89.4 % of 50 mg L⁻¹ beta-cp within 7 days. The optimal conditions for beta-cp degradation were determined to be 34.5 °C, pH 6.7, and inocula amount 0.11 g dry wt L⁻¹ using response surface methodology. The kinetic parameters q ₘₐₓ, K ₛ, and K ᵢ were established to be 2.19 day⁻¹, 76.37 mg L⁻¹, and 54.14 mg L⁻¹, respectively. The critical inhibitor concentration was determined to be 64.30 mg L⁻¹. Seven metabolites were identified by gas chromatography–mass spectrometry. Furthermore, a novel biodegradation pathway for beta-cp was proposed on the basis of analysis of the metabolites. This strain was also capable of degrading a wide range of pyrethroid insecticides including cypermethrin, deltamethrin, cyhalothrin, and beta-cyfluthrin, which similar to beta-cp are hazardous chemicals. Taken together, our results depict the biodegradation pathway of beta-cp and highlight the promising potentials of strain BSF01 in bioremediation of pyrethroid-contaminated environments.
Agid:
1214556