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A High Density Consensus Genetic Map of Tetraploid Cotton That Integrates Multiple Component Maps through Molecular Marker Redundancy Check

Author:
Blenda, Anna, Fang, David D., Rami, Jean-François, Garsmeur, Olivier, Luo, Feng, Lacape, Jean-Marc, Zhang, Jinfa
Source:
PLoS ONE 2012 v.7 no.9
ISSN:
1932-6203
Subject:
Gossypium barbadense, Gossypium hirsutum, Gossypium raimondii, allopolyploidy, chromosome mapping, cotton, diploidy, evolution, genes, genetic markers, loci, microsatellite repeats, molecular cloning, quantitative trait loci, tetraploidy
Abstract:
A consensus genetic map of tetraploid cotton was constructed using six high-density maps and after the integration of a sequence-based marker redundancy check. Public cotton SSR libraries (17,343 markers) were curated for sequence redundancy using 90% as a similarity cutoff. As a result, 20% of the markers (3,410) could be considered as redundant with some other markers. The marker redundancy information had been a crucial part of the map integration process, in which the six most informative interspecific Gossypium hirsutum × G. barbadense genetic maps were used for assembling a high density consensus (HDC) map for tetraploid cotton. With redundant markers being removed, the HDC map could be constructed thanks to the sufficient number of collinear non-redundant markers in common between the component maps. The HDC map consists of 8,254 loci, originating from 6,669 markers, and spans 4,070 cM, with an average of 2 loci per cM. The HDC map presents a high rate of locus duplications, as 1,292 markers among the 6,669 were mapped in more than one locus. Two thirds of the duplications are bridging homoeologous A T and D T chromosomes constitutive of allopolyploid cotton genome, with an average of 64 duplications per A T /D T chromosome pair. Sequences of 4,744 mapped markers were used for a mutual blast alignment (BBMH) with the 13 major scaffolds of the recently released Gossypium raimondii genome indicating high level of homology between the diploid D genome and the tetraploid cotton genetic map, with only a few minor possible structural rearrangements. Overall, the HDC map will serve as a valuable resource for trait QTL comparative mapping, map-based cloning of important genes, and better understanding of the genome structure and evolution of tetraploid cotton.
Agid:
1216132