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Isolation and HPLC method development of azafrin from Alectra parasitica var. chitrakutensis

Agrawal, Poonam, Laddha, Kirti, Tiwari, Ashok
Natural product research 2014 v.28 no.13 pp. 940-944
Alectra, Scrophulariaceae, carotenoids, infrared spectroscopy, isolation techniques, mass spectrometry, nuclear magnetic resonance spectroscopy, quantitative analysis, reversed-phase high performance liquid chromatography, rhizomes, solvents, spectral analysis, thin layer chromatography
This study was undertaken to isolate and quantify azafrin in Alectra parasitica (Scrophulariaceae) rhizomes. A simple method for the isolation of carotenoid, azafrin, involves solvent extraction of the dried rhizome powder using a single solvent and further purification by recrystallisation. The structure of the compound was elucidated and confirmed by thin-layer chromatography, infrared spectroscopy, mass spectroscopy and nuclear magnetic resonance spectral analysis. A specific and rapid reversed-phase high-performance liquid chromatography (HPLC) method was developed for the analysis of azafrin. The method was validated for accuracy, precision, linearity and specificity. Validation revealed that the method is specific, accurate, precise, reliable and reproducible. The proposed HPLC method can be used for the identification and quantitative analysis of azafrin in A. parasitica rhizomes.