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In-Vitro Simulated Gastric Fluid Digestion and Immunogenicity of Different Crustacean Protein Extracts

Wu, Li-Sha, Li, Zhen-Xing, Lu, Zong-Chao, Sun, Miao, Jamil, Khalid, Lin, Hong, Wang, Guo-Ying
International journal of food properties 2015 v.18 no.1 pp. 43-53
Acetes chinensis, Mantis, Western blotting, allergenicity, crayfish, digestion, enzyme-linked immunosorbent assay, gastric juice, immune response, in vitro digestibility, molecular weight, pepsin, polyacrylamide gel electrophoresis, shrimp, tropomyosins
To compare the immunogenicity and digestibility in-vitro simulated gastric fluid, the protein extracts of white-leg shrimp, Chinese shrimp (female and male shrimp were separated), Southern rough shrimp, Acetes chinensis , Mantis shrimp and crawfish were analyzed by SDS-PAGE, indirect ELISA and Western blotting. The results showed that except for Acetes chinensis , the larger proportion of molecular mass were near to 25 kDa (sarcoplasmic calcium-binding protein) and 38 kDa (tropomyosin). White-leg shrimp protein and Southern rough shrimp protein had a maximum similarity of 68.2%. White-leg shrimp protein had higher immunogenicity than others. There were much more IgE-binding bands in male Chinese shrimp protein, while Crawfish just had only one IgE-binding band (38 kDa, tropomyosin). In simulated gastric fluid system, the protein such as 25 kDa was rapidly degraded within 0.5 mins, while tropomyosin (38 kDa) was relatively resistant to pepsin digestion. The Western blotting results indicated that the allergenicity of those crustacean protein extracts still remained even they were digested with simulated gastric fluid for 60 min.