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Carrageenan analysis. Part 2: Quantification in swine-adapted infant formula

Author:
Blakemore, William R., Kwok, S. Karina, Harding, Niles I.
Source:
Food additives & contaminants 2014 v.31 no.10 pp. 1670-1672
ISSN:
1944-0057
Subject:
carrageenan, food additives, good laboratory practices, infant formulas, piglets
Abstract:
An LC-MS/MS method was validated in accordance with 21CFR Part 58 Good Laboratory Practice for Non-clinical Laboratory Studies to measure the concentration of carrageenan in dose formulations used in a 28-day piglet dietary feeding study of swine-adapted infant formulations stabilised with carrageenan. Carrageenan concentrations in the test formulations were 0, 300, 1000 and 2250 mg kg –¹ formula. The method for the measurement of carrageenan was LC-MS/MS coupled with ESI in negative-ion mode for detection. Linearity was established over the range 1.00–7.50 µg ml –¹. Carrageenan dose formulation samples ranging from 0 to 2250 mg kg –¹ of carrageenan were diluted to within the linearity range for measurement.
Agid:
1227777