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Boron nutrition of cultured tobacco BY-2 cells. VII. Rapid induction of metabolic dysfunction in cells deprived of boron as revealed by microarray analysis

Author:
Koshiba, Taichi, Kobayashi, Masaru, Matsuoka, Ken, Fujiwara, Toru, Matoh, Toru
Source:
Soil science and plant nutrition 2013 v.59 no.2 pp. 189-194
ISSN:
1747-0765
Subject:
Nicotiana tabacum, boron, cell death, cell walls, complementary DNA, crosslinking, microarray technology, nutrition, pectins, temporal variation, tetrazolium, tobacco
Abstract:
In plants, boron (B) works to build the cell wall structure by forming borate ester cross-links with pectin, and the deficiency of this element brings about oxidative damage and cell death. However, the process via which B deficiency leads to such catastrophe has not been well understood. In this study, we investigated the temporal changes in transcript profiles of B-deprived cultured tobacco (Nicotiana tabacum L.) BY-2 cells using cDNA microarray. The results suggest that the cells deprived of B up-regulated their antioxidative defense system to cope with the stress condition. However, the adaptive responses seemed to have declined within the first 12 h of deprivation. Tetrazolium reducing activity of B-deprived cells was lower than that of control cells at 12 h. These results together suggest that cellular function in B-deprived cells was seriously damaged as early as 12 h after deprivation. The rapidity of the onset of B deprivation-induced damages suggests the importance of the pectic network covalently cross-linked with B.
Agid:
1232145