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Trace metal concentrations and antioxidant activity in ovarian tissue of blue crab Callinectes amnicola from Lagos lagoon and implications for reproductive success

Olakolu, Fisayo Christie, Chukwuka, Azubuike Victor
Zoology and ecology 2014 v.24 no.3 pp. 278-284
Callinectes, antioxidant activity, antioxidants, cadmium, catalase, copper, crabs, discriminant analysis, dry season, ecology, enzyme activity, glutathione, glutathione peroxidase, lead, malondialdehyde, prediction, superoxide dismutase, wet season, zinc, zoology
Trace metal residues (Zn, Pb, Cd and Cu) and antioxidant enzyme activity (superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase, reduced glutathione and malondialdehyde and total protein) were measured across seasons in the ovarian tissue of blue crabs Callinectes amnicola from the Lagos lagoon areas receiving industrial discharges and from the unpolluted mid-lagoon (reference site). The independent t -test showed higher concentrations (p < 0.05) of Cd and Zn in the ovarian tissue of blue crabs from polluted areas compared to the reference site during the dry season and rainy season. The step-wise discriminant analysis distinguished between the ovarian tissues of blue crabs from polluted and reference sites with a prediction accuracy of 85.7% (p < 0.001) with GPx and SOD maximizing discrimination between the groups. The higher discriminant scores of GPx and SOD from the polluted site (33.70, 20.94) compared to those from the reference site (28.75, 16.63) reflect heightened antioxidant activity in the ovarian tissue of crabs from the polluted site, respectively. The higher levels of antioxidant activity in the ovarian tissue of the crabs from the polluted site implicates levels of metal uptake which may impair cellular functions and consequently affect reproductive outcomes of C. amnicola in similarly polluted areas of the Lagos lagoon.