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Isolation and Characterization of Antimony-Reducing Bacteria from Sediments Collected in the Vicinity of an Antimony Factory

Author:
Nguyen, Van Khanh, Lee, Jong-Un
Source:
Geomicrobiology journal 2014 v.31 no.10 pp. 855-861
ISSN:
1521-0529
Subject:
Sinorhizobium, acetates, antimony, bacteria, carbon, magnesium, nucleotide sequences, oxidation, oxygen, pH, phylogeny, ribosomal RNA, sediments, sodium, temperature, Korean Peninsula
Abstract:
To date, there is very little information available on the oxidation and reduction of antimony by bacteria. An antimonate-respiring bacterium was isolated from sediment samples collected in the vicinity of an antimony oxide-producing factory in Korea. This bacterium was isolated by application of the Hungate standard anaerobic culture technique. Temporarily named strain JUK-1, it was found to be a rod-shaped bacterium and occurred individually or in pairs. Antimonate (pentavalent antimony) was reduced to antimonite (trivalent antimony) in the presence of JUK-1 in an anoxic minimal medium containing 5 mM antimonate and 10 mM acetate. The organism grew optimally at an initial pH of 7.7 and a temperature of 30°C. A part of the antimonite which was produced in medium precipitated as a bio-mineral containing approximately 50.0% antimony, 33.5% oxygen, 12.7% carbon, 2.0% sodium and 1.9% magnesium by weight. Based on the phylogenetics of 16S rRNA gene sequence and DNA G+C content, JUK-1 appears to be a new strain of the Sinorhizobium genus. The results suggest that bacteria may play a significant role in changing the redox state of antimony in the environment.
Agid:
1233940