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Bosea vaviloviae sp. nov., a new species of slow-growing rhizobia isolated from nodules of the relict species Vavilovia formosa (Stev.) Fed.

Safronova, Vera I., Kuznetsova, Irina G., Sazanova, Anna L., Kimeklis, Anastasiia K., Belimov, Andrey A., Andronov, Evgeny E., Pinaev, Alexander G., Chizhevskaya, Elena P., Pukhaev, Andrey R., Popov, Konstantin P., Willems, Anne, Tikhonovich, Igor A.
Antonie van Leeuwenhoek 2015 v.107 no.4 pp. 911-920
Bosea, Lathyrus latifolius, Vavilovia, essential genes, fatty acids, internal transcribed spacers, new species, phylogeny, ribosomal DNA, root nodules, sequence homology, slow-growing strains, Russia
The Gram–negative, rod-shaped slow-growing strains Vaf-17, Vaf-18ᵀand Vaf-43 were isolated from the nodules of Vavilovia formosa plants growing in the hard-to-reach mountainous region of the North Ossetian State Natural Reserve (north Caucasus, Russian Federation). The sequencing of 16S rDNA (rrs), ITS region and five housekeeping genes (atpD, dnaK, recA, gyrB and rpoB) showed that the isolated strains were most closely related to the species Bosea lathyri (class Alphaproteobacteria, family Bradyrhizobiaceae) which was described for isolates from root nodules of Lathyrus latifolius. However the sequence similarity between the isolated strains and the type strain B. lathyri LMG 26379ᵀfor the ITS region was 90 % and for the housekeeping genes it was ranged from 92 to 95 %. All phylogenetic trees, except for the rrs-dendrogram showed that the isolates from V. formosa formed well-separated clusters within the Bosea group. Differences in phenotypic properties of the B. lathyri type strain and the isolates from V. formosa were studied using the microassay system GENIII MicroPlate BioLog. Whole-cell fatty acid analysis showed that the strains Vaf-17, Vaf-18ᵀand Vaf-43 had notable amounts of C₁₆:₀(4.8–6.0 %), C₁₆:₀3-OH (6.4–6.6 %), C₁₆:₁ω5c (8.8–9.0 %), C₁₇:₀cyclo (13.5–13.9 %), C₁₈:₁ω7c (43.4–45.4 %), C₁₉:₀cyclo ω8c (10.5–12.6 %) and Summed Feature (SF) 3 (6.4–8.0 %). The DNA–DNA relatedness between the strains Vaf-18ᵀand B. lathyri LMG 26379ᵀwas 24.0 %. On the basis of genotypic and phenotypic analysis a new species Bosea vaviloviae sp. nov. (type strain RCAM 02129ᵀ = LMG 28367ᵀ = Vaf-18ᵀ) is proposed.