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Interleukin/chitosan (JY) adjuvant enhances the mucosal immunity of human papillomavirus 16 L1 virus-like particles in mice

Author:
Ma, Fenlian, Zhang, Qian, Zheng, Lishu
Source:
Biotechnology letters 2015 v.37 no.4 pp. 773-777
ISSN:
0141-5492
Subject:
Human papillomavirus 16, adjuvants, chitosan, humans, immune response, immunoglobulin G, interleukin-2, lymphocytes, mice, mucosal immunity, neutralization, neutralizing antibodies, respiratory system, vaccines, virus-like particles
Abstract:
Mucosal immunity may provide a defense against human papillomavirus (HPV) but there are no FDA-approved adjuvants capable of stimulating immune responses within mucosal tissues. After mice were immunized intranasally three times with HPV16 L1 virus-like particles plus with JY adjuvant, which is composed of interleukin-2 and chitosan, sera IgG antibody titer, sera neutralizing antibody titer, sIgA concentration in respiratory tract washes, sIgA concentration in vaginal washes and the number of spot-forming cells (SFC) in splenic lymphocytes were 320 ± 15, 40 ± 2, 27 ± 1.3, 27 ± 1.7 μg/ml and 176.7 ± 6 SFC/10⁶, respectively; In the group without JY adjuvant, the outcomes were 80 ± 9.4, null, 22 ± 1, 20 ± 2.4 μg/ml and 91 ± 5.2 SFC/10⁶, respectively. Therefore, JY adjuvant may be an effective mucosal adjuvant for HPV vaccine in mice.
Agid:
1237146