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Production methods for viral particles
- Machida, Kodai, Imataka, Hiroaki
- Biotechnology letters 2015 v.37 no.4 pp. 753-760
- capsid, chemical engineering, coat proteins, drugs, virus assembly, virus-like particles
- Viral particles and virus-like particles (VLPs) or capsids are becoming important vehicles and templates in bio-imaging, drug delivery and materials sciences. Viral particles are prepared by infecting the host organism but VLPs are obtained from cells that express a capsid protein. Some VLPs are disassembled and then re-assembled to incorporate a material of interest. Cell-free systems, which are amenable to manipulating the viral assembly process, are also available for producing viral particles. Regardless of the production system employed, the particles are functionalized by genetic and/or chemical engineering. Here, we review various methods for producing and functionalizing viral particles and VLPs, and we discuss the merits of each system.