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BoLA-DRB3 gene polymorphism and FMD resistance or susceptibility in Wanbei cattle

Lei, Wei, Liang, Qinglong, Jing, Luo, Wang, Chengmin, Wu, Xiaobing, He, Hongxuan
Molecular biology reports 2012 v.39 no.9 pp. 9203-9209
Foot-and-mouth disease virus, alleles, amino acid substitution, amino acids, cattle, disease resistance, evolution, exons, foot-and-mouth disease, genotype, hydrophobicity, phosphorylation, polymerase chain reaction, protective effect, protein kinase C, protein structure, proteins, restriction endonucleases, restriction fragment length polymorphism, sequence analysis, signal transduction, translation (genetics)
For the further characterization of foot-and-mouth disease virus (FMDV)-induced foot-and-mouth disease, we investigated the association between polymorphism of BoLA-DRB3 gene and FMD resistance/susceptibility of Wanbei cattle challenged with FMDV. One hundred cattle were challenged with FMDV and exon 2 of BoLA-DRB3 genes was amplified by hemi-nested polymerase chain reaction from asymptomatic animals and from animals with FMD. PCR products were characterized by the RFLP technique using restriction enzymes Hae III. The results revealed extensive polymorphisms, 6 RFLP patterns were identified. By analyzing alleles and genotypic frequencies between healthy and infection with FMD cattle, we found that allele Hae III A was associated with susceptibility to FMD in Wanbei cattle (P < 0.05), whereas Hae III C was associated with resistance to FMD (P < 0.01) and may have a strong protective effect against FMD. Hae IIICC and Hae III BC genotype were associated with resistance to FMD (P < 0.01). By contrast, Hae III AA genotype was associated with susceptibility to FMD (P < 0.01). Sequence analysis show that 89 amino acids were translated in exon 2 of BoLA-DRB3 and 13.70 % of nucleotide mutated, which resulted in 14.61 % of amino acid change. One PKC, one Tyr and one CAMP phosphorylation were increased; the hydrophobicity and secondary structure of proteins produced change after amino acid substitution. These results revealed that Wanbei cattle had the ability of resistance to disease by mutation which result changes of the protein structure to perform the regulation of the cell using different signaling pathways in the long process of choice evolution.