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Sphingobacterium yamdrokense sp. nov., isolated from Lake Yamdrok

Author:
Xiao, Na, Liu, Yongqin, Gu, Zhengquan, Liu, Xiaobo, Jiao, Nianzhi, Liu, Hongcan, Zhou, Yuguang, Shen, Liang
Source:
Antonie van Leeuwenhoek 2015 v.107 no.5 pp. 1331-1336
ISSN:
0003-6072
Subject:
DNA, Sphingobacterium, fatty acids, isoprenoids, lakes, menaquinones, nucleotide sequences, pH, phosphatidylethanolamines, phylogeny, psychrophilic bacteria, ribosomal RNA, sodium chloride, China
Abstract:
A strictly aerobic and facultatively psychrophilic bacterium, strain 3-0-1ᵀ, was isolated from Lake Yamdrok on the Tibetan Plateau, China. Cells were Gram-negative and short-rod-shaped. Strain 3-0-1ᵀ formed circular, opaque, yellow colonies, and grew at 0–5 % (w/v) NaCl, pH 5.0–11.0 and 5–35 °C. Phylogenetic analysis based on 16S rRNA gene sequences of various species with validly published names showed that strain 3-0-1ᵀ belongs to the genus Sphingobacterium, family Sphingobacteriaceae, sharing the highest similarity with Sphingobacterim nematocida M-SX103ᵀ (96.62 %) and less than 95 % similarity with all the other species in the genus Sphingobactrium. The predominant isoprenoid quinone of strain 3-0-1ᵀ was identified as menaquinone 7 (MK-7), the major polar lipid was identified as phosphatidylethanolamine and the DNA G+C content was determined to be 42.5 mol%. The predominant cellular fatty acids were identified as summed feature 3 (C₁₆:₁ ω7c and/or C₁₆:₁ ω6c) (>30 %), iso-C₁₅:₀ (>20 %) and iso-C₁₇:₀ 3-OH (>10 %). Based on the variability of phylogenetic and phenotypic characteristics, strain 3-0-1ᵀ represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium yamdrokense sp. nov. is proposed. The type strain is 3-0-1ᵀ (=JCM 19397ᵀ = CGMCC 1.12560ᵀ).
Agid:
1239725