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Arenimonas taoyuanensis sp. nov., a novel bacterium isolated from rice-field soil in China

Zhang, Shi-Ying, Xiao, Wei, Xia, Yun-Sheng, Wang, Yong-Xia, Cui, Xiao-Long, Zhang, Nai-Ming
Antonie van Leeuwenhoek 2015 v.107 no.5 pp. 1181-1187
DNA, bacteria, chemotaxonomy, fatty acids, nucleotide sequences, pH, phosphatidylethanolamines, phylogeny, ribosomal RNA, sodium chloride, soil, ubiquinones, villages, China
A Gram-stain negative, aerobic, rod-shaped bacterial strain, YN2-31Aᵀ, was isolated from rice-field soil, Taoyuan Village, Yunnan province of China. The bacterium was observed to grow at 20–45 °C (optimum 28 °C), at pH 5.0–10.0 (optimum 7.0), and in the presence of 0–2 % (w/v) NaCl (optimum 0–1 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YN2-31Aᵀ is most closely related to Arenimonas daejeonensis DSM 18060ᵀ (96.1 %), Arenimonas malthae DSM 21305ᵀ (95.9 %), Arenimonas donghaensis DSM 18148ᵀ (95.1 %), Arenimonas composti DSM 18010ᵀ (94.8 %) and Arenimonas maotaiensis JCM 19710ᵀ (94.8 %). The major cellular fatty acids (>10 %) were found to be iso-C₁₈:₁ ω9c, iso-C₁₅:₀, Sum In Feature 3 (C₁₆:₁ ω7c/C₁₆:₁ ω6c), and C₁₆:₀. The major ubiquinone was identified as Q-8 and the major cellular polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified phospholipids. The genomic DNA G+C content was determined to be 72.3 mol%. The results of the phylogenetic, genetic, phenotypic and chemotaxonomic analyses suggest that strain YN2-31Aᵀ represents a novel species of the genus Arenimonas, for which the name Arenimonas taoyuanensis sp. nov. is proposed. The type strain is YN2-31Aᵀ (=DSM 26777ᵀ = CCTCC AB2012964ᵀ).