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Engineered single-chain variable fragment antibody for immunodiagnosis of groundnut bud necrosis virus infection

Author:
Maheshwari, Yogita, Vijayanandraj, S., Jain, R. K., Mandal, Bikash
Source:
Archives of virology 2015 v.160 no.5 pp. 1297-1301
ISSN:
0304-8608
Subject:
tomatoes, hybridomas, peanuts, Escherichia coli, cowpeas, serodiagnosis, Watermelon bud necrosis tospovirus, antibodies, serotypes, Groundnut bud necrosis tospovirus, clones, mung beans, Solanum lycopersicum var. lycopersicum, India
Abstract:
Few studies have been done on engineered antibodies for diagnosis of tospovirus infections. The present study was undertaken to develop a single-chain variable fragment (scFv) for specific diagnosis of infection by groundnut bud necrosis virus (GBNV), the most prevalent serogroup IV tospovirus in India. Heavy chain (372 nucleotide [nt]) and light chain (363 nt) variable region clones obtained from a hybridoma were used to make an scFv construct that expressed a ~29-kDa protein in E. coli. The scFv specifically detected GBNV in field samples of cowpea, groundnut, mung bean, and tomato, and it did not recognize watermelon bud necrosis virus, a close relative of GBNV belonging to tospovirus serogroup IV. This study for the first time demonstrated the application of a functional scFv against a serogroup-IV tospovirus.
Agid:
1243391