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Isolation, molecular characterization and evaluation of the pathogenicity of a porcine rotavirus isolated from Jiangsu Province, China

Author:
Zhang, Hewei, Zhang, Zhuo, Wang, Yufei, Wang, Xin, Xia, Mingqi, Wu, Hua
Source:
Archives of virology 2015 v.160 no.5 pp. 1333-1338
ISSN:
0304-8608
Subject:
Rotavirus, cytopathogenicity, diarrhea, feces, genotype, genotyping, piglets, viral load, virulent strains, China
Abstract:
In this study, a porcine rotavirus was isolated from a fecal sample from a diarrheic piglet in Jiangsu Province, China. Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 10⁶.¹²⁵TCID₅₀/ml. By applying an 80 % nucleotide cutoff value and the RotaC².⁰automated genotyping tool, the Vp4 genotype of the new isolate was identified as P[7]. The Vp7 genotype was identified as G[9], lineage VI, and sublineage c. Experimentally infected piglets showed severe diarrhea symptoms 16–24 h post-inoculation, indicating that this new porcine rotavirus isolate is a pathogenic strain.
Agid:
1243402