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Isolation, molecular characterization and evaluation of the pathogenicity of a porcine rotavirus isolated from Jiangsu Province, China
- Zhang, Hewei, Zhang, Zhuo, Wang, Yufei, Wang, Xin, Xia, Mingqi, Wu, Hua
- Archives of virology 2015 v.160 no.5 pp. 1333-1338
- Rotavirus, cytopathogenicity, diarrhea, feces, genotype, genotyping, piglets, viral load, virulent strains, China
- In this study, a porcine rotavirus was isolated from a fecal sample from a diarrheic piglet in Jiangsu Province, China. Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 10⁶.¹²⁵TCID₅₀/ml. By applying an 80 % nucleotide cutoff value and the RotaC².⁰automated genotyping tool, the Vp4 genotype of the new isolate was identified as P. The Vp7 genotype was identified as G, lineage VI, and sublineage c. Experimentally infected piglets showed severe diarrhea symptoms 16–24 h post-inoculation, indicating that this new porcine rotavirus isolate is a pathogenic strain.