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Development and Characterization of Chloroplast Microsatellite Markers in A Fine-Leaved Fescue, Festuca rubra (Poaceae)

Author:
Cräutlein, Maria von, Korpelainen, Helena, Helander, Marjo, Väre, Henry, Saikkonen, Kari
Source:
Applications in plant sciences 2014 v.2 no.12
ISSN:
2168-0450
Subject:
Festuca rubra, alleles, breeding, chloroplast genome, chloroplasts, coevolution, endophytes, genetic markers, grasses, microsatellite repeats, plant breeders, population, taxonomy, Faroe Islands, Finland, Greenland, Norway, Spain
Abstract:
Premise of the study: Chloroplast microsatellite markers were developed for Festuca rubra to examine its population genetic characteristics, taxonomy, and coevolution with its endophyte Epichloë festucae. Methods and Results: Thirteen polymorphic markers were identified from the chloroplast genome of a F. ovina accession and intergenic chloroplast sequences of F. rubra accessions. They amplified a total of 65 alleles in a sample of 93 individuals of F. rubra originating from six different populations located in the Faroe Islands, Finland, Greenland, Norway, and Spain. Conclusions: The developed microsatellite primer pairs can be used by researchers in population genetic and taxonomic studies, and by plant breeders in breeding programs on grasses.
Agid:
1249434