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Evidence for cyclin A and cyclin B genes in the ovary of the horseshoe crab, Tachypleus tridentatus: molecular cloning and expression profiles

Li, Wenxing, Huang, Huiyang, Huang, Jingru, Ye, Haihui
Marine and freshwater behaviour and physiology 2015 v.48 no.3 pp. 163-176
Tachypleus tridentatus, arthropods, complementary DNA, cyclins, genes, molecular cloning, nucleotide sequences, oocytes, oogenesis, oogonia, phylogeny
The horseshoe crab Tachypleus tridentatus is an ancient marine animal often referred to as a living fossil. Two full-length cDNA sequences were isolated from the ovary of T. tridentatus, and evidence was found that they are cyclin A (designated as TTCyA) and cyclin B (TTCyB). This is the first evidence of their presence in the Class Merostomata. The putative TTCyA cDNA was 3001 and TTCyB 1545 bp. The results of comparison and phylogenetic analysis suggest that the two sequences may have changed little in the horseshoe crab but have undergone rapid evolution in later arthropod groups. During the ovarian maturation cycle, the transcripts of TTCyA and TTCyB in the ovary increased significantly from the oogonia stage to the vitellogenic oocyte stage (p < 0.05). The results imply that both sequences are related to oogenesis in the horseshoe crab.