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Effects of Duration of Cold Storage of Host Eggs on Percent Parasitism and Adult Emergence of Each of Ten Trichogrammatidae (Hymenoptera) Species

Spínola-Filho, Paulo Roberto de Carvalho, Leite, Germano Leão Demolin, Soares, Marcus Alvarenga, Alvarenga, Anarelly Costa, Paulo, Paula Daiana de, Tuffi-Santos, Leonardo David, Zanuncio, José Cola
The Florida entomologist 2014 v.97 no.1 pp. 14-21
Ephestia kuehniella, Trichogramma, Trichogrammatoidea, adults, biological control, cold storage, cost effectiveness, eggs, mass rearing, natural enemies, parasitism, parasitoids, storage time
Improving parasitoid mass rearing techniques is important to reduce costs of biological control programs and supply natural enemies at times of high demand. The objective of this study was to evaluate the suitability of Anagasta kuehniella (Lepidoptera: Pyralidae) eggs stored at 5 °C for different time periods as a host for Trichogrammatoidea annulata (Hymenoptera: Trichogrammatidae) and for 9 Trichogramma species (T. acacioi, T. atopovirilia, T. benneti, T. brasiliensis, T. bruni, T. demoraesi, T. galloi, T. pretiosum, and T. soaresi). The experiment had a factorial design with 10 parasitoid species, 9 host storage periods (5, 10, 15, 20, 25, 30, 35 and 40 days, and a no storage control) and 20 replications, each consisting of one card (0.4 × 2.0 cm) with 40.70 ± 0.03 eggs of A. kuehniella. Trichogrammatoidea annulata, T. acacioi, T. brasiliensis, T. bruni, T. pretiosum, and T. soaresi parasitized eggs of A. kuehniella stored up to 24 days; T. atopovirilia parasitized eggs stored up to 16 days. Trichogramma demoraesi and T. benneti parasitized eggs stored for 15 days and T. galloi parasitized eggs stored for up to 13 days. The percentage of parasitized eggs decreased when the storage period increased. Among the tested parasitoids T. acacioi parasitized eggs stored for longer periods and showed the highest percentage both of parasitism and adult emergence.