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Direct plant regeneration of curry leaf tree (murraya koenigii koenig.), an aromatic plant
- ROUT, GYANA RANJAN
- In vitro cellular & developmental biology 2005 v.41 no.2 pp. 133-136
- plantlets, sucrose, acclimation, trees, rooting, benzyladenine, naphthaleneacetic acid, in vitro regeneration, Bergera koenigii, indole acetic acid, shoots
- An efficient protocol for plant regeneration from stem segments of Murraya koenigii was developed by culturing on Murashige and Skoog (MS) medium supplemented with 2.5 mg l⁻⁻¹ benzyladenine (BA), 25 mg l⁻⁻¹ adenine sulfate, 0.25 mg l⁻⁻¹ indole-3-acetic acid (IAA), and 3%% sucrose. The frequency of shoot bud regeneration was higher on similar medium in subsequent subcultures. The regenerated shoots were rooted on half-strength basal MS medium supplemented with 0.25––0.5 mg l⁻⁻¹ IAA or 1-naphthaleneacetic acid (NAA) within 8––12 d of culture. The maximum percentage of rooting was obtained on MS medium supplemented with IAA and NAA, each at 0.25 mg l⁻⁻¹. During acclimatization, 95%% of rooted plantlets survived and were grown normally under greenhouse conditions.