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Effects of growth regulators and genotypes on pyrethrum in vitro Plant
- OBUKOSIA, SILAS D., KIMANI, ESTHER, WAITHAKA, KIMANI, MUTITU, EUNICE, KIMANI, P. M.
- In vitro cellular & developmental biology 2005 v.41 no.2 pp. 162-166
- 2,4-D, Tanacetum cinerariifolium, benzyladenine, callus, genotype, naphthaleneacetic acid, shoots
- The effects of growth regulators on culture response of different pyrethrum (Chrysanthemum cinerariaefolium Vis.) genotypes were investigated. In the genotype Sb/66/107, the presence of 2,4-dichorophenoxyacetic acid (2,4-D) at 2 mg l⁻⁻¹ promoted growth of callus, whereas benzyladenine and 1-naphthaleneacetic acid had no effect. Callus growth was also affected by the 2,4-D concentration and genotypes. The optimal callus growth for the nine pyrethrum genotypes varied widely in response to 2,4-D, ranging from 0.5 mg l⁻⁻¹ for genotype Marwanga to 3.0 mg l⁻⁻¹ for Ks/75/336. Among the genotypes, shoots were regenerated from calluses of Sb/66/107, 4331, Marwanga, and MA/70/1013.