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Role of antioxidants and amino acids on somatic embryogenesis of pinus patula Plant
- MALABADI, RAVINDRA B., VAN STADEN, JOHANNES
- In vitro cellular & developmental biology 2005 v.41 no.2 pp. 181-186
- Pinus patula, amino acids, antioxidants, dithiothreitol, explants, genotype, plantlets, shoots, somatic embryogenesis, trees
- This study investigated the role of antioxidants and amino acids on somatic embryogenesis using the vegetative shoot apices of three genotypes of mature Pinus patula trees. Apart from dithiothreitol at 0.1%%, pretreatment of explants or incorporation of antioxidants in the basal nutrient medium had a negative effect on the initiation of embryogenic cultures, somatic embryo production, and plantlet recovery. Inclusion of an amino acid solution mixture, at a concentration of 10.0 mM, in the maturation medium tended to increase somatic embryo production.