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Micropropagation of pseudoxytenanthera stocksii munro Plant
- SANJAYA,, RATHORE, T. S., RAVISHANKAR RAI, V.
- In vitro cellular & developmental biology 2005 v.41 no.3 pp. 333-337
- additives, ascorbic acid, benzyladenine, citric acid, cysteine, genotype, glutamine, indole butyric acid, light intensity, micropropagation, naphthaleneacetic acid, photoperiod, shoots, tillers
- An efficient and reproducible procedure for the large-scale propagation of Pseudoxytenanthera stocksii is described. High-frequency multiple shoot induction was achieved from nodal shoot segments collected from superior/elite genotypes on Murashige and Skoog (MS) liquid medium supplemented with 1-naphthaleneacetic acid (NAA; 2.68 μμM) and 6-benzylaminopurine (BA; 4.40 μμM) at 28 ±± 1°°C and 60 μμmol m⁻⁻² s⁻⁻¹ light intensity under 12 h photoperiod. In vitro-differentiated shoots were multiplied on MS liquid medium fortified with NAA (2.68 μμM), BA (2.21 μμM) and additives: ascorbic acid (283.93 μμM), citric acid (118.10 μμM), cysteine (104.04 μμM), and glutamine (342.24 μμM). Subculturing was carried out every 2 wk on fresh shoot multiplication medium. About 125––150 shoots per culture flask were harvested within 45––50 d. In vitro-differentiated shoot clumps (three or four shoots) were successfully rooted on half-strength MS basal liquid medium with indole-3-butyric acid (4.90 μμM), BA (0.44 μμM), and additives. This is the first report where in vitro-and in vivo- (through tillers) raised clonal plants were acclimatized and established in the field, where they exhibited normal growth.