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Micropropagation of terminalia bellirica roxb. –– a sericulture and medicinal plant Plant

Author:
RAMESH, M., UMATE, PAVAN, VENUGOPAL RAO, K., SADANANDAM, A.
Source:
In vitro cellular & developmental biology 2005 v.41 no.3 pp. 320-323
ISSN:
1054-5476
Subject:
Terminalia bellirica, benzyladenine, explants, indole butyric acid, kinetin, medicinal plants, micropropagation, rooting, seedlings, sericulture, shoots, woody plants
Abstract:
A protocol for micropropagation of plants via axillary bud proliferation from nodal explants of Terminalia bellirica Roxb. seedlings has been established. Explants were cultured on Murashige and Skoog medium with different concentrations of 6-benzyladenine (BA; 4.4, 8.9, 13.3, 17.8, or 22.2 μμM) or kinetin (Kn; 4.6, 9.3, 14.0, 18.6, or 23.2 μμM). Within the range evaluated, the medium containing 13.3 μμM BA showed the highest shoot length (1.9 ±± 0.2 cm) in the primary culture. When separated and transferred to fresh subculture medium with lower levels of BA (2.2, 4.4, 6.6, or 8.9 μμM) or Kn (2.3, 4.6, 6.9, or 9.3 μμM), the nodal segments from individual regenerants (obtained initially from seedling nodes) showed efficient shoot induction at 4.4 μμM BA. Rooting of the shoots was achieved under in vitro conditions on two media tested, i.e., modified Gamborg's (B₅) medium or Woody Plant Medium, both supplemented with 4.9 μμM indole-3-butyric acid. Regenerated plants were established in the greenhouse.
Agid:
1279696