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Tissue culture and plant regeneration of the salt marsh monocots juncus roemerianus and juncus gerardi Plant
- WANG, JIANGBO, SELISKAR, DENISE M., GALLAGHER, JOHN L.
- In vitro cellular & developmental biology 2005 v.41 no.3 pp. 274-280
- 2,4-D, Juncus roemerianus, benzyladenine, callus, coconut water, greenhouse soils, indole acetic acid, indole butyric acid, inflorescences, naphthaleneacetic acid, planting, rooting, salt marsh plants, salt marshes, seeds, shoots, thidiazuron, tissue culture
- Tissue culture and plant regeneration protocols for the salt marsh plants Juncus roemerianus Scheele and Juncus gerardi Loisel. were developed. J. roemerianus callus was induced from mature seeds cultured on Murashige and Skoog (MS) medium supplemented with 2.22 μμM 6-benzylaminopurine (BA), 5.37 μμM αα-naphthaleneacetic acid (NAA), 2.26 μμM 2,4-dichlorophenoxyacetic acid (2,4-D), and 50 ml l⁻⁻¹ coconut water (callus induction medium). The callus was subcultured on MS medium containing 2.22 μμM BA, 5.37 μμM NAA, and 9.05 μμM 2,4-D for callus maintenance. Shoot regeneration occurred 2 wk after transferring the callus onto shoot regeneration medium, which consisted of MS medium containing BA or thidiazuron. A high frequency of shoot regeneration was obtained when the medium contained 13.3 μμM BA. Regenerated shoots were transferred to MS medium supplemented with 10.7 μμM NAA for root production. Rooting did not occur in the shoots regenerated on the thidiazuron-containing media. The callus induction medium for J. roemerianus was also effective in inducing callus of J. gerardi from young inflorescences. The same medium was also used for callus maintenance. Shoot regeneration occurred 10 d after transferring the callus onto MS medium supplemented with 0.44 μμM BA and 0.57 μμM indole-3-acetic acid. Root regeneration occurred after transferring the shoots onto MS medium plus 0.44 μμM BA and 14.8 μμM indole-3-butyric acid. The regenerated plants of both J. roemerianus and J. gerardi grew vigorously in potting soil in the greenhouse. J. roemerianus regenerants also grew well in a saltwater-irrigated field plot. Tissue culture-produced plants of J. roemerianus and J. gerardi can be used for planting in created or restored wetlands.