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AN efficient micropropagation system for eclipta alba –– a valuable medicinal herb Plant

Author:
BASKARAN, P., JAYABALAN, N.
Source:
In vitro cellular & developmental biology 2005 v.41 no.4 pp. 532-539
ISSN:
1054-5476
Subject:
Eclipta prostrata, benzyladenine, carbon, coconut water, gardens, gibberellic acid, indole butyric acid, isopentenyladenine, kinetin, medicinal plants, micropropagation, plantlets, rooting, sand, shoots, soil, sucrose
Abstract:
An efficient rapid and large-scale in vitro clonal propagation of the valuable medicinal herb Eclipta alba (Asteraceae) by enhanced axillary shoot proliferation in cotyledonary node segments was designed. The medium type, various carbon sources, plant growth regulators, and coconut water markedly influenced in vitro propagation of Eclipta alba. An in vitro plantlet production system has been investigated on Murashige and Skoog (MS) medium with the synergistic combination of benzyladenine (4.4 μμM), kinetin (4.6 μμM), 2-isopentenyladenine (4.9 μμM), gibberellic acid (1.4 μμM), 5%% coconut water, and 3%% sucrose which promoted the maximum number of shoots as well as beneficial shoot length. Subculturing of cotyledonary node segments on a similar medium enabled continuous production of healthy shoots with similar frequency. Rooting was highest (94.3%%) on full strength MS medium containing 9.8 μμM indolebutyric acid. Micropropagated plants established in garden soil, farmyard soil, and sand (2:1:1) were uniform and identical to the donor plant with respect to growth characteristics as well as floral features. These plants grew normally without showing any morphological variation.
Agid:
1279728