Jump to Main Content
In vitro regeneration and plant establishment of tylophora indica (burm. f.) merrill: petiole callus culture Plant
- FAISAL, M., SINGH, S., ANIS, M.
- In vitro cellular & developmental biology 2005 v.41 no.4 pp. 511-515
- 2,4-D, Tylophora indica, callus, callus culture, culture media, explants, gardens, greenhouses, indole butyric acid, petioles, plant establishment, plantlets, roots, shoots, soil, thidiazuron, vermiculite
- A protocol has been developed for high-frequency shoot regeneration and plant establishment of Tylophora indica from petiole-derived callus. Optimal callus was developed from petiole explants on Murashige and Skoog basal medium supplemented with 10 μμM 2,4-dichlorophenoxyacetic acid ++2.5 μμM thidiazuron (TDZ). Adventitious shoot induction was achieved from the surface of the callus after transferring onto shoot induction medium. The highest rate (90%%) of shoot multiplication was achieved on MS medium containing 2.5 μμM TDZ. Individual elongated shoots were rooted best on half-strength MS medium containing 0.5 μμM indole-3-butyric acid (IBA). When the basal cut ends of the in vitro-regenerated shoots were dipped in 150 μμM IBA for 30 min followed by transplantation in plastic pots containing sterile vermiculite, a mean of 4.1 roots per shoot developed. The in vitro-raised plantlets with well-developed shoot and roots were successfully established in earthen pots containing garden soil and grown in a greenhouse with 100%% survival. Four months after transfer to pots, the performance of in vitro-propagated plants of T. indica was evaluated on the basis of selected physiological parameters and compared with ex vitro plants of the same age.