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Ontogeny of somatic embryos in cucumber (cucumis sativus l) Plant
- VENGADESAN, G., SELVARAJ, N., ANAND, R. PREM, GABA, V., GANAPATHI, A.
- In vitro cellular & developmental biology 2005 v.41 no.6 pp. 789-793
- 2,4-D, Cucumis sativus, activated carbon, benzyladenine, callus, cucumbers, gibberellic acid, ontogeny, photoperiod, plant growth, somatic embryos, sucrose
- Suspension culture of cucumber (Cucumis sativus L.) has been an inefficient method for production of somatic embryos owing to problems with embryo maturation and conversion. Embryogenic callus of cv. Green Long was induced on semi-solid Murashige and Skoog (MS) medium containing 6.8 μμM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.2 μμM 6-benzylaminopurine (BA). A large number of globular somatic embryos were obtained on transfer of the callus to MS liquid medium supplemented with 87.6 mM sucrose, 1.1 μμM 2,4-D, and improved by the addition of 342.4 μμM l-glutamine. MS medium supplemented with 87.6 mM sucrose was more effective in somatic embryo production than other sugars. Subsequent development led to the formation of heart- and torpedo-shaped embryos. Maturation of somatic embryos occurred on plant growth regulator-free MS semi-solid medium containing 175.2 mM sucrose and 0.5 g l⁻⁻¹ activated charcoal. Conversion of embryos into plants was achieved on half-strength MS semi-solid medium containing 87.6 mM sucrose and 1.4 μμM gibberellic acid (GA₃) in a 16 h photoperiod. Twenty-seven percent of embryos were converted into normal plants.