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Cryopreservation and plant regeneration via somatic embryogenesis using shoot apical domes of mature pinus roxburghii sarg. trees Plant
- MALABADI, RAVINDRA B., NATARAJA, K.
- In vitro cellular & developmental biology 2006 v.42 no.2 pp. 152-159
- Pinus roxburghii, abscisic acid, cryopreservation, cryoprotectants, dimethyl sulfoxide, gellan gum, plantlets, shoots, somatic embryogenesis, somatic embryos, sorbitol, trees
- The present investigation reports optimized parameters for somatic embryogenesis and cryopreservation of embryogenic cultures using shoot apical domes from mature trees of Pinus roxburghii Sarg. Embryogenic tissue of P. roxburghii Sarg. was cryopreserved for 24 h, 10 d, and 8 wk using sorbitol and dimethylsulfoxide (DMSO) as cryoprotectants. Results indicate that 0.2 M sorbitol and 5%% DMSO had the best cryoprotecting effect. The recovered tissue showed luxuriant growth on maintenance medium (II). Partial desiccation of thawed embryogenic tissue for 24 h prior to transfer to maturation medium enhanced the maturation of somatic embryos. Maturation frequency increased from 1.3 to 18.3%% after 12 h desiccation treatment, and from 18.3 to 61.8%% after 24 h of desiccation. However, non-desiccated embryogenic tissue produced the least number of somatic embryos (1.3%%) on the maturation medium with the same abscisic acid and Gellan gum concentration. All the three embryogenic lines produced plantlets and had the same appearance and normal growth as compared to unfrozen controls.