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Rhizobium yantingense sp. nov., a mineral-weathering bacterium

Author:
Chen, Wei, Sheng, Xia-Fang, He, Lin-Yan, Huang, Zhi
Source:
International journal of systematic and evolutionary microbiology 2015 v.65 no.Pt 2 pp. 412-417
ISSN:
1466-5034
Subject:
Rhizobium huautlense, bacteria, essential genes, fatty acids, flagellum, nucleotide sequences, phylogeny, ribosomal RNA, sequence homology, sulfur dioxide, China
Abstract:
A Gram-stain-negative, rod-shaped bacterial strain, H66 ᵀ, was isolated from the surfaces of weathered rock (purple siltstone) found in Yanting, Sichuan Province, PR China. Cells of strain H66 ᵀ were motile with peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain H66 ᵀ belongs to the genus Rhizobium . It is closely related to Rhizobium huautlense SO2 ᵀ (98.1 %), Rhizobium alkalisoli CCBAU 01393 ᵀ (98.0 %) and Rhizobium cellulosilyticum ALA10B2 ᵀ (98.0 %). Analysis of the housekeeping genes, recA , glnII and atpD , showed low levels of sequence similarity (<92.0 %) between strain H66 ᵀ and other recognized species of the genus Rhizobium . The predominant components of the cellular fatty acids were summed feature 8 (C ₁₈ : ₁ω7 c and/or C ₁₈ : ₁ω6 c) and C ₁₆ : ₀. The G+C content of strain H66 ᵀ was 60.3 mol%. Strain H66 ᵀ is suggested to be a novel species of the genus Rhizobium based on the low levels of DNA–DNA relatedness (ranging from 14.3 % to 40.0 %) with type strains of species of the genus Rhizobium and on its unique phenotypic characteristics. The name http://dx.doi.org/10.1601/nm.1279 Rhizobium yantingense sp. nov. is proposed for this novel species. The type strain is H66 ᵀ (= CCTCC AB 2014007 ᵀ = LMG 28229 ᵀ).
Agid:
1281480