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Burkholderia susongensis sp. nov., a mineral-weathering bacterium isolated from weathered rock surface

Gu, Jia-Yu, Zang, Sheng-Gang, Sheng, Xia-Fang, He, Lin-Yan, Huang, Zhi, Wang, Qi
International journal of systematic and evolutionary microbiology 2015 v.65 no.Pt 3 pp. 1031-1037
Burkholderia, DNA, bacteria, fatty acids, genes, mica, pH, phosphatidylethanolamines, phylogeny, ribosomal RNA, sodium chloride, China
A novel type of mineral-weathering bacterium was isolated from the weathered surface of rock (mica schist) collected from Susong (Anhui, China). Cells of strain L226 ᵀ were Gram-stain-negative. The strain grew optimally at 30 °C, with 1 % (w/v) NaCl and at pH 7.0 in trypticase soy broth. On the basis of 16S rRNA gene phylogeny, strain L226 ᵀ was shown to belong to the genus Burkholderia and the closest phylogenetic relatives were Burkholderia sprentiae WSM5005 ᵀ (98.3 %), Burkholderia acidipaludis NBRC 101816 ᵀ (98.2 %), Burkholderia tuberum STM678 ᵀ (97.2 %) and Burkholderia diazotrophica JPY461 ᵀ (97.1 %). The DNA G+C content was 63.5 mol% and the respiratory quinone was Q-8. The major fatty acids were C ₁₆ : ₀, C ₁₇ : ₀ cyclo and C ₁₉ : ₀ cyclo ω8 c . The polar lipid profile of strain L226 ᵀ consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, unknown lipids and unidentified aminophospholipids. Based on the low level of DNA–DNA relatedness (ranging from 25.8 % to 34.4 %) to the tested type strains of species of the genus Burkholderia and unique phenotypic characteristics, it is suggested that strain L226 ᵀ represents a novel species of the genus Burkholderia , for which the name Burkholderia susongensis sp. nov., is proposed. The type strain is L226 ᵀ (= CCTCC AB2014142 ᵀ = JCM 30231 ᵀ).