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Catenisphaera adipataccumulans gen. nov., sp. nov., a member of the family Erysipelotrichaceae isolated from an anaerobic digester

Author:
Kanno, Manabu, Katayama, Taiki, Morita, Naoki, Tamaki, Hideyuki, Hanada, Satoshi, Kamagata, Yoichi
Source:
International journal of systematic and evolutionary microbiology 2015 v.65 no.Pt 3 pp. 805-810
ISSN:
1466-5034
Subject:
DNA, Firmicutes, alanine, anaerobic digesters, bacteria, chemotaxonomy, fatty acids, fermentation, galactose, glucose, glutamic acid, lysine, nucleotide sequences, pH, peptidoglycans, phospholipids, phylogeny, rhamnose, ribose, ribosomal RNA, sequence homology, serine, threonine, yeast extract, Japan
Abstract:
An obligately anaerobic bacterium, designated strain GK12 ᵀ, was isolated from an anaerobic digester in Fukagawa, Hokkaido Prefecture, Japan. The cells of strain GK12 ᵀ were non-motile, non-spore-forming cocci that commonly occurred in chains. 16S rRNA gene sequence analysis revealed that strain GK12 ᵀ was affiliated with the family Erysipelotrichaceae in the phylum Firmicutes and showed 91.8 % sequence similarity to the most closely related species, Faecalicoccus acidiformans . The strain grew at 30–50 °C (optimally at 40 °C) and at pH 5.5–8.5 (optimally at pH 7.5). The main end product of glucose fermentation was lactate. Yeast extract was required for growth. The strain contained C ₁₄ : ₀, C ₁₄ : ₀ 1,1-dimethoxyalkane (DMA), C ₁₆ : ₀ DMA and C ₁₈ : ₀ DMA as the major cellular fatty acids (>10 % of the total). The polar lipid profile was composed of phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid. The whole-cell sugars were galactose, rhamnose and ribose. The cell-wall murein contained alanine, glutamic acid, lysine, serine and threonine, but not diaminopimelic acid. The G+C content of the genomic DNA was 47.7 mol%. Based on phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Catenisphaera adipataccumulans gen. nov., sp. nov., is proposed to accommodate strain GK12 ᵀ (= NBRC 108915 ᵀ = DSM 25799 ᵀ).
Agid:
1281555