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Catenisphaera adipataccumulans gen. nov., sp. nov., a member of the family Erysipelotrichaceae isolated from an anaerobic digester

Kanno, Manabu, Katayama, Taiki, Morita, Naoki, Tamaki, Hideyuki, Hanada, Satoshi, Kamagata, Yoichi
International journal of systematic and evolutionary microbiology 2015 v.65 no.Pt 3 pp. 805-810
DNA, Firmicutes, alanine, anaerobic digesters, bacteria, chemotaxonomy, fatty acids, fermentation, galactose, glucose, glutamic acid, lysine, nucleotide sequences, pH, peptidoglycans, phospholipids, phylogeny, rhamnose, ribose, ribosomal RNA, sequence homology, serine, threonine, yeast extract, Japan
An obligately anaerobic bacterium, designated strain GK12 ᵀ, was isolated from an anaerobic digester in Fukagawa, Hokkaido Prefecture, Japan. The cells of strain GK12 ᵀ were non-motile, non-spore-forming cocci that commonly occurred in chains. 16S rRNA gene sequence analysis revealed that strain GK12 ᵀ was affiliated with the family Erysipelotrichaceae in the phylum Firmicutes and showed 91.8 % sequence similarity to the most closely related species, Faecalicoccus acidiformans . The strain grew at 30–50 °C (optimally at 40 °C) and at pH 5.5–8.5 (optimally at pH 7.5). The main end product of glucose fermentation was lactate. Yeast extract was required for growth. The strain contained C ₁₄ : ₀, C ₁₄ : ₀ 1,1-dimethoxyalkane (DMA), C ₁₆ : ₀ DMA and C ₁₈ : ₀ DMA as the major cellular fatty acids (>10 % of the total). The polar lipid profile was composed of phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid. The whole-cell sugars were galactose, rhamnose and ribose. The cell-wall murein contained alanine, glutamic acid, lysine, serine and threonine, but not diaminopimelic acid. The G+C content of the genomic DNA was 47.7 mol%. Based on phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Catenisphaera adipataccumulans gen. nov., sp. nov., is proposed to accommodate strain GK12 ᵀ (= NBRC 108915 ᵀ = DSM 25799 ᵀ).