Main content area

Choreography of the Mycobacterium Replication Machinery during the Cell Cycle

Trojanowski, Damian, Ginda, Katarzyna, Pióro, Monika, Hołóówka, Joanna, Skut, Partycja, Jakimowicz, Dagmara, Zakrzewska-Czerwióóńska, Jolanta
mBio 2015 v.6 no.1 pp. e02125-14
Escherichia coli, Mycobacterium smegmatis, bacteria, chromosome segregation, chromosomes, fluorescence microscopy
It has recently been demonstrated that bacterial chromosomes are highly organized, with specific positioning of the replication initiation region. Moreover, the positioning of the replication machinery (replisome) has been shown to be variable and dependent on species-specific cell cycle features. Here, we analyzed replisome positions in Mycobacterium smegmatis , a slow-growing bacterium that exhibits characteristic asymmetric polar cell extension. Time-lapse fluorescence microscopy analyses revealed that the replisome is slightly off-center in mycobacterial cells, a feature that is likely correlated with the asymmetric growth of Mycobacterium cell poles. Estimates of the timing of chromosome replication in relation to the cell cycle, as well as cell division and chromosome segregation events, revealed that chromosomal origin-of-replication ( oriC ) regions segregate soon after the start of replication. Moreover, our data demonstrate that organization of the chromosome by ParB determines the replisome choreography.