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Adsorption of a porcine reproductive and respiratory syndrome virus DNA vaccine candidate onto biodegradable nanoparticles improves immunogenicity in mice

Author:
Du, Luping, Li, Bin, Xu, Xiangwei, Sun, Bing, Pang, Fengjiao, Wen, Libin, Huang, Kehe, He, Kongwang
Source:
Archives of virology 2015 v.160 no.6 pp. 1543-1547
ISSN:
0304-8608
Subject:
DNA viruses, Porcine reproductive and respiratory syndrome virus, adsorption, biodegradability, cell-mediated immunity, genes, immune response, mice, models, nanoparticles, plasmids, porcine reproductive and respiratory syndrome, recombinant vaccines, swine, vaccine development
Abstract:
Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating viral disease of pigs. Safer and more effective vaccines are urgently needed. In this study, a synthetic ORF5 gene of porcine reproductive and respiratory syndrome virus (PRRSV) was adsorbed onto poly(D, L-lactide-co-glycolide)/polyethylenimine (PLGA/PEI) nanoparticles. We prepared a PLGA-nanoparticle-adsorbed PRRSV DNA vaccine and a PEI-DNA complex. The results showed that these model vaccines could significantly enhance humoral and cellular immune responses when compared with the responses induced by pcDNA3.1-SynORF5, a plasmid construct for expression of PRRSV ORF5. PLGA-branched PEI nanoparticles induced the most efficient immune response. The delivery system and adjuvant provide new models for the development of vaccines against PRRSV.
Agid:
1330372